戴建英   

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Language:English

Paper Publications

Title of Paper:玉米皮固态培养混合益生菌的研究

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Date of Publication:2016-04-20

Journal:中国饲料

Included Journals:PKU

Issue:8

Page Number:25-28,42

ISSN No.:1004-3314

Key Words:玉米皮;固态培养;纤维素酶;蛋白酶;益生菌

Abstract:本试验以玉米皮为主要原料,通过单因素试验和正交试验对黑曲霉与酿酒酵母的混合固态培养进行了研究。以蛋白酶、纤维素酶、活菌数为主要考察指标,优化得出的最佳培养条件为:尿素添加量0.5%(P<0.05)、含水量60%、麸皮添加量15%(P<0.05)、黑曲霉接种量20%、培养天数7 d;混菌培养接种比(黑曲霉∶酿酒酵母)为3∶1,混菌共培养1 d。在此条件下,纤维素酶的酶活为622.11 U/g,蛋白酶的酶活为606.55 U/g,每克发酵物含益生活菌总数超过百亿。

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