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    戴建英

    • 副教授     博士生导师   硕士生导师
    • 性别:女
    • 毕业院校:新墨西哥大学
    • 学位:博士
    • 所在单位:生物工程学院
    • 学科:生物化工. 微生物学
    • 办公地点:生物工程学院307
    • 联系方式:jydai@dlut.edu.cn
    • 电子邮箱:jydai@dlut.edu.cn

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    AI-2 Key Enzyme S-Ribosylhomocysteinase from Strain Klebsiella pneumoniae CICC 10011 Producing 2,3-Butanediol

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    论文类型:期刊论文

    发表时间:2011-03-01

    发表刊物:CHEMICAL RESEARCH IN CHINESE UNIVERSITIES

    收录刊物:Scopus、SCIE、ISTIC

    卷号:27

    期号:2

    页面范围:273-276

    ISSN号:1005-9040

    关键字:S-Ribosylhomocysteinase(LuxS); Kinetics; Characterization; Klebsiella pneumoniae

    摘要:S-Ribosylhomocysteinase(LuxS) is the key enzyme in the synthetic pathway of a quorum sensing autoinducer AI-2. LuxS from a 2,3-butanediol produced strain Klebisella pneumoniae CICC 10011 was cloned and characterized. The luxS gene is composed of 540 bp with 172 amino acids encoded. The K(m) value for S-ribosylhomocysteine(SRH) was (27 +/- 1) mmol/L, k(cat) was (0.112 +/- 0.004) s(-1) and k(cat) /K(m) was 4.4x10(3) L(.)mol(-1.)s(-1) at 25 degrees C. LuxS was activated by divalent metal ions, the highest activity was detected with Co(2+) form, followed by Mg(2+), Ba(2+), Mn(2+), Fe(2+) and Ca(2+), and activation constant for Co(2+) is (16 +/- 2) mu mol/L.