个人信息Personal Information
副教授
硕士生导师
性别:女
毕业院校:北京大学
学位:博士
所在单位:化工海洋与生命学院
学科:药剂学
电子邮箱:guozm@dlut.edu.cn
The Improved Delivery to Breast Cancer Based on a Novel Nanocarrier Modified with High-Affinity Peptides Discovered by Phage Display
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论文类型:期刊论文
发表时间:2018-10-24
发表刊物:ADVANCED HEALTHCARE MATERIALS
收录刊物:PubMed、SCIE
卷号:7
期号:20
页面范围:e1800269
ISSN号:2192-2640
关键字:breast cancer; cell-binding peptides; cell-internalizing peptides; ligand-targeted nanosystems; phage display
摘要:Ligand-targeted nanosystems have the potential to realize site-specific tumor therapy and alleviate unwanted side effects of many chemotherapeutic agents, and one of the most key issues seems to be the construction of an effective nanocarrier. Based on different processes of phage display techniques, 38 cell-binding peptides and 32 cell-internalizing peptides are discovered. Four of these ligand peptides [FIPFDPMSMRWE (FIP), NASSFPTNSRWA (NAS), GLHTSATNLYLH (GLH), and ALAVAPSRWWNE (ALA), respectively] exhibit high affinity to MCF7 human breast cancer cells. Among them, NAS and ALA are reported for the first time, whose affinities are 20.6 and 76.3 times that of the random peptide control, respectively. Both NAS and ALA modifications to doxorubicin-loaded lipid nanosytems [LP(DOX)] show stronger tumor inhibition, longer animal survival time, and less body weight loss, compared to unmodified or control peptide modified nanosystems, on an MCF7 tumor-bearing mouse model. In conclusion, the cell-binding peptide NAS and cell-internalizing peptide ALA can be used for ligand-targeted delivery of antitumor drugs. It seems that the in vivo antitumor effect of these ligand-targeted nanosystems is closely related to their ligand-cell affinity, but fairly tolerant of the ligand types.
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