Release Time:2019-03-12  Hits:

Indexed by: Journal Article

Date of Publication: 2018-06-01

Journal: MICROCHIMICA ACTA

Included Journals: SCIE、PubMed

Volume: 185

Issue: 6

Page Number: 291

ISSN: 0026-3672

Key Words: Manganese dioxide/carbon based dots nanohybrids; Oxidation; Tetramethylbenzidine; Fatel calf serum sample; Visual detection

Abstract: A kind of single-layer carbon based dots (CDs) with abundant carboxyl functional groups was hybridized with manganese dioxide (MnO2). The resulting nanohybrid is stable and can be well dispersed in water. MnO2 is capable of oxidizing the substrate 3,3'5,5'-tetramethylbenzidine (TMB) to form a blue product whose absorption (peaking at 655 nm) fades in the presence of glutathione (GSH). A sensitive and selective colorimetric GSH assay was worked out that has a linear response in the 10 to 0.1 mu M GSH concentration range, with a 0.095 mu M detection limit. The method was applied to the determination of GSH in spiked fetal calf serum where it gave excellent recoveries.