点击次数：

论文类型：期刊论文

发表时间：2016-09-15

发表刊物：BIOSENSORS & BIOELECTRONICS

收录刊物：SCIE、EI、PubMed、Scopus

卷号：83

页面范围：193-199

ISSN号：0956-5663

关键字：NIR fluorescent probe; Carboxylesterase 2; Enzymatic activity; Specific detection; Bioimaging

摘要：A near-infrared fluorescent probe (DDAB) for highly selective and sensitive detection of carboxylesterase 2 (CE2) has been designed, synthesized, and systematically studied both in vitro and in vivo. Upon addition of CE2, the ester bond of DDAB could be rapidly cleaved and then release a near-infrared (NIR) fluorophore DDAO, which brings a remarkable yellow-to-blue color change and strong NIR fluorescence emission in physiological solutions. The newly developed probe exhibits excellent properties including good specificity, ultrahigh sensitivity and high imaging resolution. Moreover, DDAB has been applied to measure the real activities of CE2 in complex biological samples, as well as to screen CE2 inhibitors by using tissue preparations as the enzymes sources. The probe has also been successfully used to detect endogenous CE2 in living cells and in vivo for the first time, and the results demonstrate that such detection is highly reliable. All these prominent features of DDAB make it holds great promise for further investigation on CE2-associated biological process and for exploring the physiological functions of CE2 in living systems. (C) 2016 Elsevier B.V. All rights reserved.