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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：男
毕业院校：日本冈山大学
学位：博士
所在单位：化工学院
学科：精细化工. 应用化学
办公地点：大连理工大学西部校区E209
联系方式：13591789942
电子邮箱：jncui@dlut.edu.cn
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A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity
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论文类型：期刊论文
发表时间：2021-01-29
发表刊物：CHEMICAL COMMUNICATIONS
卷号：53
期号：48
页面范围：6448-6451
ISSN号：1359-7345
摘要：A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O-6-benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.