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论文类型：期刊论文

发表时间：2014-12-15

发表刊物：SENSORS AND ACTUATORS B-CHEMICAL

收录刊物：SCIE、Scopus

卷号：205

页面范围：151-157

ISSN号：0925-4005

关键字：Human carboxylesterase 2; Ratiometric fluorescent probe; 4-Hydroxy-N-butyl-1,8-naphthalimide; Bioimaging

摘要：A new ratiometric fluorescent probe derived from 4-hydroxy-N-butyl-1,8-naphthalimide (HNN) has been developed for selective detection of human carboxylesterase 2 (hCE2). The probe is designed by introducing benzoyl moiety to HNN, based on the intramolecular charge transfer (ICT) mechanism. The probe displays satisfying stability under physiological pH conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hCE2 and release of HNN which leads to a remarkable red shift in emission spectra (148 nm). The newly designed probe exhibits excellent selectivity towards hCE2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. Its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hCE2 in human living cells, have also been demonstrated. (C) 2014 Elsevier B.V. All rights reserved.