欢迎对环境分析、生物分析、化学传感、功能核酸感兴趣的学生加入刘猛院长团队！！！微信：l570896990，邮箱：lijiuxing@dlut.edu.cn。

个人简介：

李久兴，大连理工大学环境学院副教授，博士生导师、硕士生导师，入选国家博士后海外引才专项。从事生物传感、环境分析等领域研究，具体包括核酸适配体筛选、生物传感器件构建等，致力于病毒、细菌、蛋白等环境与医学相关靶标的精准分析。以第一/通讯作者发表论文30余篇，包括Journal of the American Chemical Society（1篇）、Angewandte Chemie International Edition（6篇）、Environmental Science & Technology（1篇）、Nucleic Acids Research (1篇)等，总被引3100余次，H指数28。获得授权国家发明专利7项，申请国际专利6项。担任Biocontaminant、Biosensors、Sensors期刊编委。

教育与工作经历:

2007-09 至 2011-06, 厦门大学, 化学生物学, 学士

2011-09 至 2016-06, 厦门大学, 分析化学, 博士（导师：杨朝勇教授）

2016-10 至 2017-10, 美国，密歇根理工大学（导师：夏小虎教授）

2017-12 至 2024-11, 加拿大，麦克马斯特大学（导师：李应福教授）

2024-12 至今, 大连理工大学，环境学院，副教授 （刘猛院长团队）

代表性科研项目:

国家博士后海外引才专项，2025.10-2027.10，90万，主持

国家自然科学基金面上项目，2026.01-2029.12，50万，主持

中央高校基本科研业务费，2024.12-2027.12，40万，主持

代表性第一/通讯作者论文：

(1)   Xue, W.; Sheng, T.; Chang, Y.; Li, X.; Jin, R.; Zhang, Q.; Yuan, H.; Li, Z.; Zhang, R.; Zhang, Z.; Li, Jiuxing^*; Liu, M.^* DNAzyme-Based Core–Shell Hydrogel Microneedle Arrays for In Situ Live Bacterial Detection on Surfaces. Environ. Sci. Technol. 2026, acs.est.6c04666. https://doi.org/10.1021/acs.est.6c04666.

(2)   Xue, W.; Li, R.; Wang, K.; Song, K.; Zhang, Z.; Li, Jiuxing^*; Chang, Y.^*; Liu, M.^* Deep Learning-Enhanced DNAzyme-Driven Rolling-Circle Amplification Encoding for Multibacterial Detection. Angew. Chem. Int. Ed. 2026, 65 (22), e4446117. https://doi.org/10.1002/anie.4446117.

(3)   Wu, Y.; Jin, R.; Lei, T.; Liu, J.; Chang, Y.; Zhang, Z.; Li, Jiuxing^*; Liu, M.^* Aptamer-Coupled Droplet CRISPR/Cas12a Enables Ultrasensitive sPD-L1 Detection. Anal. Chem. 2026, 98 (22), 16296–16305. https://doi.org/10.1021/acs.analchem.6c00779.

(4)   Li, Jiuxing; Liu, R.; Zhang, W.; Salena, B. J.; Li, Y. A Portable Colorimetric Device for Rapid Bacterial Detection with Cleavable Functional Nucleic Acid Probes for A Common Bacterial Endoribonuclease. Angew. Chem. Int. Ed. 2026, e5320160. https://doi.org/10.1002/anie.5320160.

(5)   Wang, F.; Meng, Q.; Huang, Z.; Ren, Y.; Zhang, Z.; Chang, Y.; Zhang, R.; Li, Y.; Li, Jiuxing^*; Liu, M.^* Recent Advances in Aptamer-Based Biosensors for Viral Detection. Biocontaminant 2025, 1 (1), e020. https://doi.org/10.48130/biocontam-0025-0018.

(6)   Li, X.; Jia, P.; Xiao, S.; Zhang, Z.; Xue, D.; Chang, Y.; Zhang, R.; Zhang, Q.; Li, S.; Liu, R.; Qu, C.; Li, Jiuxing^*; Liu, M.^* DNAzyme-Based Point-of-Care Diagnosis of Aggressive Periodontitis. ACS Sens. 2025, 10 (11), 9077–9085. https://doi.org/10.1021/acssensors.5c03632.

(7)   Wang, Q.^#; Li, Jiuxing^#; Zhang, Z.; Amini, R.; Derdall, A.; Gu, J.; Xia, J.; Salena, B. J.; Yamamura, D.; Soleymani, L.; Li, Y. Fighting Mutations with Mutations: Evolutionarily Adapting a DNA Aptamer for High-Affinity Recognition of Mutated Spike Proteins of SARS-CoV-2. Angew. Chem. Int. Ed. 2024, 63, e202415226. https://doi.org/10.1002/anie.202415226.

(8)   Rudi Liu^#; Li, Jiuxing ^#; Jimmy Gu; Bruno J. Salena; Yingfu Li, Palladium–iridium nanocubes modified with a high-affinity DNA aptamer as paired viral diagnostic and therapeutic tools. Advanced Sensor and Energy Materials 2024, 100125.

(9)   Li, Jiuxing; Zhang, Z.; Liu, R.; Amini, R.; Salena, B. J.; Li, Y. Discovery and Translation of Functional Nucleic Acids for Clinically Diagnosing Infectious Diseases: Opportunities and Challenges. TrAC Trends Anal. Chem. 2023, 158, 116886. https://doi.org/10.1016/j.trac.2022.116886.

(10) Li, Jiuxing; Khan, S.; Gu, J.; Filipe, C. D. M.; Didar, T. F.; Li, Y. A Simple Colorimetric Au-on-Au Tip Sensor with a New Functional Nucleic Acid Probe for Food-Borne Pathogen Salmonella Typhimurium. Angew. Chem. Int. Ed. 2023, 62 (20), e202300828. https://doi.org/10.1002/anie.202300828.

(11) Chang, D.^#; Li, Jiuxing^#; Liu, R.^#; Liu, M.; Tram, K.; Schmitt, N.; Li, Y. A Colorimetric Biosensing Platform with Aptamers, Rolling Circle Amplification and Urease-Mediated Litmus Test. Angew. Chem. Int. Ed. 2023, 62 (51), e202315185. https://doi.org/10.1002/anie.202315185.

(12) Li, Jiuxing; Zhang, Z.; Gu, J.; Amini, R.; Mansfield, A. G.; Xia, J.; White, D.; Stacey, H. D.; Ang, J. C.; Panesar, G.; Capretta, A.; Filipe, C. D. M.; Mossman, K.; Salena, B. J.; Gubbay, J. B.; Balion, C.; Soleymani, L.; Miller, M. S.; Yamamura, D.; Brennan, J. D.; Li, Y. Three on Three: Universal and High-Affinity Molecular Recognition of the Symmetric Homotrimeric Spike Protein of SARS-CoV-2 with a Symmetric Homotrimeric Aptamer. J. Am. Chem. Soc. 2022, 144 (51), 23465–23473. https://doi.org/10.1021/jacs.2c09870.

(13) Zhang, Z. ^#; Pandey, R. ^#; Li, Jiuxing^#; Gu, J.; White, D.; Stacey, H. D.; Ang, J. C.; Steinberg, C.-J.; Capretta, A.; Filipe, C. D. M.; Mossman, K.; Balion, C.; Miller, M. S.; Salena, B. J.; Yamamura, D.; Soleymani, L.; Brennan, J. D.; Li, Yingfu. High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva. Angew. Chem. Int. Ed. 2021, 60 (45), 24266–24274. https://doi.org/10.1002/anie.202110819.

(14) Li, Jiuxing; Zhang, Z.; Gu, J.; Stacey, H. D.; Ang, J. C.; Capretta, A.; Filipe, C. D. M.; Mossman, K. L.; Balion, C.; Salena, B. J.; Yamamura, D.; Soleymani, L.; Miller, M. S.; Brennan, J. D.; Li, Y. Diverse High-Affinity DNA Aptamers for Wild-Type and B.1.1.7 SARS-CoV-2 Spike Proteins from a Pre-Structured DNA Library. Nucleic Acids Res. 2021, 49 (13), 7267–7279. https://doi.org/10.1093/nar/gkab574.

(15) Li, Jiuxing; Gu, J.; Zhang, H.; Liu, R.; Zhang, W.; Mohammed-Elsabagh, M.; Xia, J.; Morrison, D.; Zakaria, S.; Chang, D.; Arrabi, A.; Li, Y. A Highly Specific DNA Aptamer for RNase H2 from Clostridium Difficile. ACS Appl. Mater. Interfaces 2021, 13 (8), 9464–9471. https://doi.org/10.1021/acsami.0c20277.

指导学生：

团队负责人刘猛教授代表性项目：

1、 国家重点研发计划-长江黄河等重点流域水资源与水环境综合治理，项目名称：生物性污染物精准定量在线检测技术与装备，批准号：2023YFC3205804，执行期：2023.12.1-2027.11.30

2、国家重点研发计划-大气与土壤、地下水污染综合治理，项目名称：地下水卤代新污染物电化学原位传感技术与设备研发，批准号：2023YFC3711100，执行期：2023年12月至2026年11月

3、国家自然科学基金-青年科学基金项目（A类）[原国家杰出青年科学基金项目]，项目名称：生物性污染物检测，批准号：22425602，执行期：2025.1.1-2029.12.31

4、教育部学科突破先导项目，项目名称：水生态环境智慧韧性学科突破先导项目，批准号：JYB2025XDXM908，执行期：2025.9.1-2030.8.30

团队负责人刘猛教授近3年代表性论文：

(1)   Xue, W.; Li, R.; Wang, K.; Song, K.; Zhang, Z.; Li, J.; Chang, Y.; Liu, M. Deep Learning-Enhanced DNAzyme-Driven Rolling-Circle Amplification Encoding for Multibacterial Detection. Angew. Chem. Int. Ed. 2026, 65 (22), e4446117. https://doi.org/10.1002/anie.4446117.

(2)   Wu, Y.; Jin, R.; Lei, T.; Liu, J.; Chang, Y.; Zhang, Z.; Li, J.; Liu, M. Aptamer-Coupled Droplet CRISPR/Cas12a Enables Ultrasensitive sPD-L1 Detection. Anal. Chem. 2026, 98 (22), 16296–16305. https://doi.org/10.1021/acs.analchem.6c00779.

(3)   Liu, J.; Tan, F.; Cao, T.; Yu, R.; Wang, Y.; Liu, M. Rapid and Accurate Detection of Perfluorooctanesulfonic Acid in Environmental Samples via Chemiresistive Sensor Integrating Molecular Imprinting and Fluorine-Fluorine Interactions. Environ. Sci. Technol. 2026, 60 (11), 8393–8403. https://doi.org/10.1021/acs.est.5c15943.

(4)   Li, X.; Xue, W.; Xiao, S.; Li, Z.; Zhao, J.; Zhang, R.; Li, J.; Zhang, Z.; Chang, Y.; Liu, M. Converting One In Vitro Selected DNA Molecule Into Two Bacteria-Responsive DNAzymes by Regulation of Reaction Conditions. Angew. Chem.-Int. Ed. 2026. https://doi.org/10.1002/anie.7611103.

(5)   Jia, P.; Chang, Y.; Li, S.; Xue, W.; Xiao, S.; Zhang, Q.; Li, J.; Wang, Y.; Zhang, Z.; Liu, M. Acidic in Vitro Selection of Metal-Specific Deoxyribozymes. Chem. Sci. 2026, 17 (11), 5554–5562. https://doi.org/10.1039/d5sc08656f.

(6)   Zhang, R.; An, C.; Li, J.; Li, X.; Zhang, Q.; Li, Q.; Li, S.; Wang, B.; Li, X.; Chang, Y.; Zhang, Z.; Liu, M. Integrating Fluorogenic DNAzymes with Photonic Crystals for Bacterial Detection on a Microfluidic Biochip. Anal. Chem. 2025, 97 (31), 16950–16956. https://doi.org/10.1021/acs.analchem.5c02447.

(7)   Xiao, S.; Chang, Y.; Jia, P.; Li, X.; Li, X.; Li, X.; Yuan, H.; Li, J.; Zhang, Z.; Liu, M. Site-Specific Recognition of Inosine-Modified RNA by Deoxyribozyme with Distant Cleavage Site. Angew. Chem.-Int. Ed. 2025, 64 (49). https://doi.org/10.1002/anie.202513587.

(8)   Liu, Y.; Wu, Y.; Liu, Y.; Zhang, Q.; Yuan, H.; Li, S.; Li, Z.; Wang, B.; Chang, Y.; Liu, M. Arrest of CRISPR-Cas12a by Nonspecific Single-Stranded DNA for Biosensing. Anal. Chem. 2025, 97 (17), 9310–9315. https://doi.org/10.1021/acs.analchem.4c07081.

(9)   Li, X.; Jia, P.; Xiao, S.; Zhang, Z.; Xue, D.; Chang, Y.; Zhang, R.; Zhang, Q.; Li, S.; Liu, R.; Qu, C.; Li, J.; Liu, M. DNAzyme-Based Point-of-Care Diagnosis of Aggressive Periodontitis. ACS Sens. 2025, 10 (11), 9077–9085. https://doi.org/10.1021/acssensors.5c03632.

(10) Jia, P.; Meng, Q.; Zhang, Q.; Zhang, R.; Zhang, Z.; Li, J.; Wang, X.; Liu, Y.; Chang, Y.; Liu, M. Engineering Acidic Deoxyribozyme with Strongly Cooperative H+ Binding for Subcellular Imaging. Anal. Chem. 2025, 97 (17), 9378–9385. https://doi.org/10.1021/acs.analchem.5c00535.

(11) Chang, Y.; Liang, Y.; Song, H.; Zhang, Q.; Yuan, H.; Li, J.; Zhang, Z.; Liu, M. Click DNA Ligation with Deoxyribozyme. NUCLEIC ACIDS Res. 2025, 53 (18). https://doi.org/10.1093/nar/gkaf991.

(12) Wu, Y.; Jin, R.; Chang, Y.; Liu, M. A High-Fidelity DNAzyme-Assisted CRISPR/Cas13a System with Single-Nucleotide Resolved Specificity. Chem. Sci. 2024, 15 (18), 6934–6942. https://doi.org/10.1039/D4SC01501K.

(13) Shi, J.; Zhang, Q.; Wu, Y.; Chang, Y.; Liu, M. In Vitro Selection of N1-Methyladenosine-Sensitive RNA-Cleaving Deoxyribozymes with 105-Fold Selectivity over Unmethylated RNA. Chem. Sci. 2024, 15 (33), 13452–13458. https://doi.org/10.1039/D4SC02943G.

(14) Li, X.; Chang, Y.; Wu, Y.; Liu, M. A DNAzymes-in-Droplets Assay for Burkholderia Gladioli Pathovar Cocovenenans with Single-Bacterium Sensitivity. Chem. Sci. 2024, 15 (8), 2996–3002. https://doi.org/10.1039/D3SC05874C.

(15) Zhou, Q.; Zhang, G.; Wu, Y.; Zhang, Q.; Liu, Y.; Chang, Y.; Liu, M. In Vitro Selection of M2+-Independent, Fast-Responding Acidic Deoxyribozymes for Bacterial Detection. J. Am. Chem. Soc. 2023, 145 (39), 21370–21377. https://doi.org/10.1021/jacs.3c06155.

(16) Yin, Q.; Zhao, D.; Chang, Y.; Liu, B.; Liu, Y.; Liu, M. Functional DNA Superstructures Exhibit Positive Homotropic Allostery in Ligand Binding. Angew. Chem. Int. Ed. 2023, 62 (25), e202303838. https://doi.org/10.1002/anie.202303838.

(17) Yan, Y.; Chang, D.; Xu, Y.; Chang, Y.; Zhang, Q.; Yuan, Q.; Salena, B. J.; Li, Y.; Liu, M. Engineering a Ligase Binding DNA Aptamer into a Templating DNA Scaffold to Guide the Selective Synthesis of Circular DNAzymes and DNA Aptamers. J. Am. Chem. Soc. 2023, 145 (4), 2630–2637. https://doi.org/10.1021/jacs.2c12666.

(18) Chang, D.; Li, J.; Liu, R.; Liu, M.; Tram, K.; Schmitt, N.; Li, Y. A Colorimetric Biosensing Platform with Aptamers, Rolling Circle Amplification and Urease-Mediated Litmus Test. Angew. Chem. Int. Ed. 2023, 62 (51), e202315185. https://doi.org/10.1002/anie.202315185.

(19) Wu, Y.; Liu, Y.; Chang, Y.; Liu, M. Integration of CRISPR/Cas13a and V-Shape PCR for Rapid, Sensitive, and Specific Genotyping of CYP2C19 Gene Polymorphisms. Anal. Chem. 2023, 95 (26), 10127–10135. https://doi.org/10.1021/acs.analchem.3c01968.

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