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个人信息Personal Information
副教授
博士生导师
硕士生导师
性别：男
毕业院校：中科院长春应用化学研究所
学位：博士
所在单位：化工学院
办公地点：大连理工大学西部校区精细化工国家重点实验室
联系方式：0411-84986251
电子邮箱：lcchen@dlut.edu.cn
Ratiometric sensing lysosomal pH in inflammatory macrophages by a BODIPY-rhodamine dyad with restrained FRET
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论文类型：期刊论文
发表时间：2021-01-10
发表刊物：CHINESE CHEMICAL LETTERS
卷号：31
期号：5
页面范围：1091-1094
ISSN号：1001-8417
关键字：pH probe; Inflammation; Lysosome; FRET; Macrophages
摘要：Inflammation, as the pathophysiological response of body to harmful stimuli, leads to changes in cellular microenvironment. To research pH changes in lysosomes of macrophages during inflammation, we designed a FRET (Forster resonance energy transfer) based probe, BDP-RhB. The probe showed good lysosome targeting ability, wide response range of pH from 8.0 to 4.0 with significant ratio (I-582/I-518) change from 0.6 to 3.4, and good reversibility and sustainability. By applying BDP-RhB, we found a decrease of lysosomal pH of macrophages during inflammation. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.