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论文类型：期刊论文

发表时间：2017-04-01

发表刊物：DYES AND PIGMENTS

收录刊物：SCIE、EI

卷号：139

页面范围：318-325

ISSN号：0143-7208

关键字：Monitoring pH; Lysosome Fluorescent probe; Cells; Ovarian tumour

摘要：Intracellular pH is important in regulating various cellular behaviour and pathological conditions. We synthesised a rhodamine-based lysosomal pH probe (hereafter referred to as RD) with a pKa of 4.10. The probe exhibited favourable "on-off' reversibility between pH 3.25 and 7.20 for at least five cycles. RD can be employed to distinguish cancer cells from normal cells on the basis of different fluorescence response. RD performed well in the detection of lysosomal pH induced by artesunate, chloroquine and dexamethasone in MCF-7 cells. The intense fluorescence of RD in ovarian tumour indicated the higher acidity in tumour tissues than that in normal tissues. Furthermore, RD worked well in the imaging of excised mouse stomach and living mice under acidic stimulation. These data demonstrate the applicability of RD in monitoring pH in complex biological systems.(C) 2016 Elsevier Ltd. All rights reserved.