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论文类型：期刊论文

发表时间：2004-12-01

发表刊物：KIDNEY INTERNATIONAL

收录刊物：SCIE、PubMed

卷号：66

期号：6

页面范围：2279-2282

ISSN号：0085-2538

关键字：intracellular mercuric ion; fluorescence; probe

摘要：Background. Imaging of intracellular mercuric ion is necessary for mechanism of renal toxicity of exposure to HgCl2. The distribution of Hg2+ inside a living cell, however, is still invisible due to the lack of high selective and sensitive fluorescent molecular probe for Hg2+.
   Methods. Anew fluorescent probe, EPNP, was applied to the cultured cells of human kidney proximal tubular epithelial cell line (HKC) in the presence of HgCl2 and some other bivalent ions. The relative fluorescence intensity of EPNP was measured and fluorescence images were taken by laser scanning confocal microscope.
   Results. Results showed it led to an Hg2+ concentration- and time-dependent increase in fluorescence intensity, and responded weakly for some other heavy and transition metal ions. It could be seen during acute exposure on HKC cells, Hg2+ locate perinuclear, and on nuclear membrane, which was beyond what one knew before.
   Conclusion. EPNP is a real-time and on-line probe for imaging Hg2+ in a living cell due to its high selectivity and sensitivity for Hg2+ and slow bleaching/fading. Both the probe and the new results about the distribution of intracellular Hg2+ may be helpful for relevant biologic research.