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个人信息Personal Information
副教授
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：化学学院
学科：有机化学
电子邮箱：liufengyu@dlut.edu.cn
A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells
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论文类型：期刊论文
发表时间：2021-04-01
发表刊物：BIOORGANIC & MEDICINAL CHEMISTRY
卷号：28
期号：3
页面范围：115280
ISSN号：0968-0896
关键字：Nitroreductase (NTR); 1,8-Naphthalimide; Escherichia coli (E. coli); Staphylococcus aureus. (S. aureus)
摘要：A nitroreductase (NTR) responsive fluorescent probe, Na-NO2, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized. Na-NO2 showed remarkable fluorescence "turn-on" signal in the presence of NTR under DMSO/H2O (1:19, v/v) buffered with PBS (pH = 7) solution in the presence of NADH (300 mu M). Furthermore, the probe has a low detection limit down to 3.4 ng/mL and it is very sensitive towards the NTR in Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), normal and tumor cells such as HL-7702, HepG-2 and MCF-7.