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论文类型：期刊论文

发表时间：2013-09-01

发表刊物：BIOMATERIALS

收录刊物：SCIE、EI、PubMed、Scopus

卷号：34

期号：27

页面范围：6473-6481

ISSN号：0142-9612

关键字：NIR dye; RNA probe; Fluorescent imaging; Large Stokes shift; Cucurbit[7]uril

摘要：A near-infrared fluorescent dye Hsd was designed and synthesized, which absorbed as hemicyane and emitted as Cy7 and therefore produced a Stokes shift as large as 224 nm. Quantum chemistry calculation demonstrated that the large Stokes shift was produced by the combination of intramolecular charge transfer (ICT) and internal conversion. Significantly, Hsd showed selectively response to RNA in aqueous solution and fixed cells. Moreover, Hsd could be uptaken into the cells under the assistance of cucurbit[7]uril (CB7) and selectively stain RNA in living cells. The introducing of CB7 provides a platform to amplify the application of some cell-impermeant fluorescent stains through the supramolecular chemistry methods. (C) 2013 Elsevier Ltd. All rights reserved.