点击次数：

论文类型：期刊论文

发表时间：2012-06-25

发表刊物：MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS

收录刊物：SCIE、EI

卷号：177

期号：11

页面范围：887-891

ISSN号：0921-5107

关键字：Single-wall carbon nanotubes (SWCNTs); Acridine orange (AO); Hybrid; DNA; Fluorescence detection

摘要：The fluorescence of acridine orange (AO) can be quenched significantly with addition of the single-wall carbon nanotubes (SWCNTs) solution, due to the formation of a hybrid complex between AO and SWCNTs. A fluorescence enhancement of approximately 18 x can be observed after the addition of certain amount of DNA into the above mentioned solution. The fluorescence increase was linearly proportional to the amount of DNA added in the concentration range of 0-50.75 mu M, and the DNA detection limit was down to 8.56 x 10(-8) M. This method can be used to detect DNA in vitro. (C) 2012 Elsevier B.V. All rights reserved.