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论文类型：期刊论文

发表时间：2014-08-31

发表刊物：Journal of Donghua University (English Edition)

收录刊物：EI、Scopus

卷号：31

期号：4

页面范围：391-395

ISSN号：16725220

摘要：Exogenous DNA expressing green fluorescent protein (GFP) and labeled with fluorescein isothiocyanate (FITC) was used to transform the Chinese oak silkmoth Antheraea pernyi(A. pernyi) via sperm-mediated gene transfer (SMGT). Sperms entry into the female reproductive system and eggs were observed using fluorescence microscopy. The ability of A. pernyi sperms to uptake exogenous DNA was confirmed, and transfer of the exogenous DNA was shown by GFP expression in the transgenic eggs. Our result suggested that SMGT could also be used to directly generate transgenic A. pernyi expressing functional genes of interest. Copyright ? 2014 Editorial Department of Journal of Donghua University.