个人信息Personal Information
副教授
博士生导师
硕士生导师
性别:男
毕业院校:大连理工大学
学位:博士
所在单位:生物工程学院
联系方式:13342277088
电子邮箱:biolwl@dlut.edu.cn
耐旱相关基因SiUPF3的克隆表达及生物信息学分析
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发表时间:2015-01-01
发表刊物:植物生理学报
卷号:51
期号:4
页面范围:509-516
ISSN号:2095-1108
摘要:Upf3 protein is one of the most important factors in the mRNA
surveillance process. This process is a conservative mRNA degradation
system which is mainly involved in the degradation of abnormal mRNA in
eukaryotic cells. To study drought-tolerant genes, we compared the
expression of Jingu34 under drought condition and normal one and
filtrated drought-tolerant genes in the level of transcription. In this
paper, we got the full length gene which is numbered Si009903m through
searching the sequence within online Setaria italica genome data.
Finally we cloned the coding sequence (CDS) of Si009903m and named it
SiUPF3 for the Upf3 protein it encoded. The CDS of Si009903m is 1 515 bp
and encodes 504 amino acids. Quantitative RT-PCR analysis infered that
SiUPF3 is regulated by ABA signal network and significantly upregulated
under drought.
备注:新增回溯数据