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论文类型：期刊论文

发表时间：2009-06-01

发表刊物：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

收录刊物：SCIE、PubMed

卷号：4

期号：2

页面范围：105-110

ISSN号：1744-117X

关键字：Antheraea pernyi; Silk glands; Two-dimensional gel electrophoresis; Glutathione S-transferase theta; Ribosomal protein L8; Real-time PCR

摘要：Antheraea pernyi is a typical wild silkworm with larval silk glands specific for the synthesis and secretion of silk proteins. We examined changes in the protein profile in the posterior section of the silk glands on days 1 and 4 of the last larval instar. Two-dimensional gel electrophoresis over the pH range of 4-7 followed by silver staining revealed about 350 protein spots. Twenty-three proteins upregulated on day 4 were analyzed using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF). Search for homologous sequences in NCBI databases identified seven proteins probably involved in the regulation of transcription, translation, and general cell metabolism. Among these, glutathione S-transferase theta (GSTT) and ribosomal protein L8 (RPL8) were selected for investigations at the mRNA level. Real-time quantitative PCR showed that the expression of GSTT peaked on day 4, whereas that of RPL8 peaked on day 3, confirming the upregulation of these proteins during the last larval instar. These results show that expressions of GSTT and RPL8 may play a role in fibroin synthesis in A. pernyi. (C) 2009 Elsevier Inc. All rights reserved.