刘军山

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研究员

博士生导师

硕士生导师

性别:男

毕业院校:大连理工大学

学位:博士

所在单位:机械工程学院

学科:机械电子工程. 机械制造及其自动化

办公地点:机械工程学院知方楼6005室

联系方式:0411-84707713

电子邮箱:liujs@dlut.edu.cn

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Culture and Differentiation of Rat Neural Stem/Progenitor Cells in a Three-Dimensional Collagen Scaffold

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论文类型:期刊论文

发表时间:2013-05-01

发表刊物:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录刊物:SCIE、EI、PubMed、Scopus

卷号:170

期号:2

页面范围:406-419

ISSN号:0273-2289

关键字:Neural stem/progenitor cells; Collagen gel; Three-dimensional culture; Neural-like tissue; Neural network

摘要:A stable and fast method for constructing a neural-like tissue from rat neural stem/progenitor cells (rNS/PCs) based on three-dimensional (3D) collagen gel is described. First step, the collagen-embedded rNS/PCs expanded with the medium consisting of DMEM/F12/RPMI1640 (1:1:1) supplemented with EGF and bFGF was used to expand the cells in gel in 96-well plates until the average diameter of cell clusters was about 50-100 mu m with the cell density higher than 10(7) cells/mL. In the second step, the initial medium was replaced with NB/B-27 supplemented with bFGF and BDNF. The results show that cells in collagen presented neural-like morphology and maintained live cell rate around 82 % in neural network pattern at least for 42 days under static conditions. The cell-collagen constructs were detected by immunofluorescence and immunohistochemistry test after 42 days of culture, part of cells still maintained the character of rNS/PCs, and others differentiated into neurons, astrocytes, and oligodendrocytes. Our 3D neural-like tissue construct was similar to the neural tissue in morphology and cell compositions. They thus have a potential to be used for drug screening, detection of environment toxins, and replacement therapy.