点击次数：

发表时间：2022-10-10

发表刊物：环境科学学报

期号：10

页面范围：3643-3650

ISSN号：0253-2468

摘要：An anthraquinone dye intermediate 1-amino-anthraquinone-2-sulfonic acid (ASA-2)-degrading strain GF3 was isolated from the bromamine acid-contaminated soil samples. Based on morphological observation and 16S rDNA sequence analysis, strain GF3 was identified as Rhodococcus pyridinivorans. The decolorization of ASA-2 by strain GF3 was studied and ASA-2 degradation product was analyzed using high performance liquid chromatography-mass spectrometry (HPLC-MS). The results show that additional peptone, yeast extract and casamino acid could promote ASA-2 bio-decolorization, among which the accelerating effect of peptone was the most obvious. Further experimental results demonstrate that many amino acids could accelerate the process of ASA-2 bio-decolorization, and L-leucine had the most significantly accelerating effect on ASA-2 decolorization and cell growth. The optimal environmental conditions for ASA-2 bio-decolorization were pH 8.0, 30 �� and 150 r��min-1. Under the optimal conditions, the decolorization efficiency of 108 mg��L-1 ASA-2 could reach over 95% in 30 h, and TOC removal rate was 62%. In UV-Visible spectra, the characteristic absorption peaks of ASA-2 totally disappeared and a new peak at 340 nm appeared when the red color of ASA-2 aqueous solution faded to be colorless. Further analysis found that the mass to charge ratio of the end product from ASA-2 degradation was 260. Thus, the degradation product was proposed to be 3-amino-4-sulfophthalic acid. In addition, strain GF3 could also degrade bromamine acid, anthraquinone-2-sulfonate and anthraquinone-2-carboxylic acid. ? 2016, Science Press. All right reserved.

备注：新增回溯数据