论文成果
Magnetic loading of tyrosinase-Fe3O4/mesoporous silica core/shell microspheres for high sensitive electrochemical biosensing
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- 论文类型：期刊论文
- 发表时间：2011-02-07
- 发表刊物：ANALYTICA CHIMICA ACTA
- 收录刊物：Scopus、SCIE、EI、PubMed
- 文献类型：J
- 卷号：686
- 期号：1-2
- 页面范围：81-86
- ISSN号：0003-2670
- 关键字：Tyrosinase; Fe3O4/mSiO(2) core/shell mesoporous; microspheres; Magnetic
loading; Flow injection analysis; Amperometry
- 摘要：A new protocol is proposed for magnetic loading and sensitive electrochemical detection of phenol via the tyrosinase cross-linked mesoporous magnetic core/shell microspheres. The mesoporous magnetic microspheres, characterized by transmission electron microscopy, N-2 adsorption/desorption isotherms, and magnetic curve displays high capacity for enzyme immobilization and strong magnetism to adhere to the magnetic electrode surface without any additional adhesive reagent. The biosensor exhibits a wide linear response to phenol ranging from 1.0 x 10(-9) to 1.0 x 10(-5) M, a high sensitivity of 78 mu A mM(-1), a low detection limit of 1 nM, and a fast response rate (less than 5 s). The proposed method is simple, rapid, inexpensive and convenient in electrode renewal, which is recommended as a promising experimental platform for wider applications in biosensing. (C) 2010 Elsevier B.V. All rights reserved.
