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Study on the extraction and back extraction of Bovine Serum Albumin using reversed micelles

发表时间:2019-03-10
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论文类型:
期刊论文
第一作者:
Li, Xiangcun
通讯作者:
Li, XC (reprint author), Dalian Univ Technol, R&D Ctr Membrane Sci & Technol, State Key Lab Fine Chem, Dalian, Peoples R China.
合写作者:
He, Gaohong,Lin, Chang,Liu, Hongjing
发表时间:
2007-12-01
发表刊物:
SEPARATION SCIENCE AND TECHNOLOGY
收录刊物:
SCIE、EI、Scopus
文献类型:
J
卷号:
42
期号:
16
页面范围:
3741-3757
ISSN号:
0149-6395
关键字:
BSA; revered micelles; extraction ratio; selectivity
摘要:
The extraction and back extraction of Bovine Serum Albumin (BSA) using a new reversed micelle system of CTAB/isooctane/1-pentanol have been investigated. Under the optimal operating conditions in this study, the extraction ratio of BSA reaches as high as 98%. The back extraction ratio can reach 80% as pH in stripping phase approaches to the isoelectric point (pI) of BSA. UV-spectra indicated that there was no significant change in the structure of BSA enriched in stripping phase. Furthermore, the reversed micelle system was repeatedly used to extract BSA from a mixed solution of protein.
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