Indexed by:
期刊论文
First Author:
Li, Xiangcun
Correspondence Author:
Li, XC (reprint author), Dalian Univ Technol, R&D Ctr Membrane Sci & Technol, State Key Lab Fine Chem, Dalian, Peoples R China.
Co-author:
He, Gaohong,Lin, Chang,Liu, Hongjing
Date of Publication:
2007-12-01
Journal:
SEPARATION SCIENCE AND TECHNOLOGY
Included Journals:
SCIE、EI、Scopus
Document Type:
J
Volume:
42
Issue:
16
Page Number:
3741-3757
ISSN No.:
0149-6395
Key Words:
BSA; revered micelles; extraction ratio; selectivity
Abstract:
The extraction and back extraction of Bovine Serum Albumin (BSA) using a new reversed micelle system of CTAB/isooctane/1-pentanol have been investigated. Under the optimal operating conditions in this study, the extraction ratio of BSA reaches as high as 98%. The back extraction ratio can reach 80% as pH in stripping phase approaches to the isoelectric point (pI) of BSA. UV-spectra indicated that there was no significant change in the structure of BSA enriched in stripping phase. Furthermore, the reversed micelle system was repeatedly used to extract BSA from a mixed solution of protein.
Translation or Not:
no
