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论文类型：期刊论文

发表时间：2013-07-01

发表刊物：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY

收录刊物：SCIE、PubMed

卷号：83

期号：3

页面范围：115-126

ISSN号：0739-4462

关键字：beta-N-acetylhexosaminidases; FDL; glycosylation; insect; N-glycan

摘要：The -N-acetylhexosaminidase FDL specifically removes the -1,2-GlcNAc residue conjugated to the -1,3-mannose residue of the core structure of insect N-glycans, playing significant physiological roles in post-translational modification in the Golgi apparatus. Little is known about its enzymatic properties. We obtained the OfFDL gene from the insect Ostrinia furnacalis by RT-PCR. The full length cDNA of FDL is 2241 bp carrying an opening reading frame of 1923 bp encoding 640 amino acids. The recombinant protein OfFDL in a soluble and active form was obtained with high purity through a two-step purification strategy. The recombinant OfFDL exclusively hydrolyzes the terminal -1,2-GlcNAc residue from the -1,3 branch instead of the -1,6 branch of the substrate GnGn-PA. Several kinetic parameters including kcat/Km values toward four artificial substrates and Ki values of three representative hexosaminidase inhibitors were obtained. (C) 2013 Wiley Periodicals, Inc.