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发表时间：2012-01-01

发表刊物：应用与环境生物学报

卷号：18

期号：5

页面范围：880-887

ISSN号：1006-687X

摘要：Aromatic oxygenases, which can catalyze the hydroxylation of aromatic
   ring, have been widely used in bioremediation and chemical synthesis. In
   this paper, the classification and application area of aromatic
   oxygenases are summarized. Moreover, their discovery strategies based on
   pure culture technology and metagenomic technology are discussed in
   detail and first evaluated. Abundant of aromatic oxygenases have been
   obtained using pure culture technology, but these enzymes exhibit with
   single type, high repetition rate and low novelty. Emerging metagenomic
   technology provides new ideas for discovering microbial resource from
   uncultured microbes. However, it is still in the initial stage of
   exploiting aromatic oxygenases by metagenomic methods, and many problems
   such as low screening efficiency and hard to express heterologously have
   not been resolved. This review evaluates the two strategies by analyzing
   the content of genetic information, screening throughout and efficiency,
   the time consume, cost, and expression feasibility of aromatic
   oxygenase. And the metagenomic methods suitable for screening different
   types of aromatic oxygenases are proposed. In the future, pure culture
   and metagenomic technology combined with other omics technologies, will
   contribute to promoting the theoretical study of aromatic oxygenases and
   rapid development of biocatalysis industry. Fig 3, Tab 2, Ref 47

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