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论文类型：期刊论文

发表时间：2015-10-01

发表刊物：BIORESOURCE TECHNOLOGY

收录刊物：SCIE、EI、PubMed、Scopus

卷号：193

页面范围：281-287

ISSN号：0960-8524

关键字：Cometabolic degradation; Carbazole; Pathway; Arthrobacter sp W1

摘要：Arthrobacter sp. W1 was used to characterize the pathways involved in cometabolic degradation of carbazole ( CA) with phenol as the primary substrate. To clarify the upper pathway of cometabolic degradation CA, Escherichia coli strain BL21 expressing phenol hydroxylase from strain W1 (PHIND) was investigated to degrade CA. Firstly, CA was initially monohydroxylated at C-2 and C-4 positions to produce 2- and 4-hydroxycarbazole, followed by successively hydroxylated to the corresponding 1,2- and 3,4-dihydroxycarbazole, of which 3,4-dihydroxycarbazole was unequivocally identified for the first time. To characterize the downstream cometabolic degradation CA pathway, purified 3,4-dihydroxycarbazole was used as the substrate for phenol-grown W1, and a series of novel indole derivatives were identified. These results suggested that a novel pathway of CA catabolism was employed by strain W1 via a successive hydroxylation and meta-cleavage pathway. These findings provide new insights into the cometabolic degradation CA process and have potential applications in biotechnology and bioremediation. (C) 2015 Elsevier Ltd. All rights reserved.