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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：环境学院
学科：环境工程. 环境科学
办公地点：环境学院B617室
联系方式：0411-84706250
电子邮箱：qyy@dlut.edu.cn
Promiscuous esterase activities of the C-C hydrolases from Dyella ginsengisoli
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论文类型：期刊论文
发表时间：2012-06-01
发表刊物：BIOTECHNOLOGY LETTERS
收录刊物：SCIE、EI、PubMed
卷号：34
期号：6
页面范围：1107-1113
ISSN号：0141-5492
关键字：C-C bond hydrolase; Esterase; Homology modeling; Molecular docking
摘要：A C-C hydrolase gene (bphD (LA-4) ) from strain Dyella ginsengisoli LA-4 was cloned and expressed in Escherichia coli BL21 (DE3). BphD(LA-4) together with another hydrolase MfphA(LA-4), which derived from the same strain, possessed esterase activities. p-Nitrophenyl butyrate was the best substrate for both enzymes. BphD(LA-4) had high catalytic efficiency to p-nitrophenyl benzoate, whereas MfphA(LA-4) had no activity. Homology modeling and docking studies demonstrated that the proper hydrogen bond interaction was important for the reactivity of specific substrate.