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论文类型：期刊论文

发表时间：2010-06-01

发表刊物：BIORESOURCE TECHNOLOGY

收录刊物：SCIE、PubMed

卷号：101

期号：12

页面范围：4527-4532

ISSN号：0960-8524

关键字：Indigo; Bio-production; Growth substrate; Response surface methodology

摘要：In this study, a novel indigo-producing bacterial strain PP-2 was isolated from activated sludge. It was identified as Acinetobacter sp. according to phylogenetic similarity of 165 rRNA gene sequence. This isolate was able to produce indigo from indole by utilizing a wide range of aromatic hydrocarbons. The results of SDS-PAGE analysis showed that the enzyme system induced by phenol was more abundant than that induced by other aromatic hydrocarbons. And the effects of metal ions on indigo production were also investigated, which indicated that the activity of cells induced with phenol could be inhibited by 0.5 mM Fe3+. Response surface methodology (RSM) was applied to optimize the process of indigo bio-production. The results exhibited that the maximal yield was achieved with 157.92 mg L-1 phenol and 205.32 mg L-1 indole. Under the optimal conditions, the indigo yield and transformation efficiency of indole were 202.92 mg L-1 and 88%, respectively. 2010 Elsevier Ltd. All rights reserved.