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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：环境学院
学科：环境工程. 环境科学
办公地点：环境学院B617室
联系方式：0411-84706250
电子邮箱：qyy@dlut.edu.cn
Enzyme-substrate interaction and characterization of a 2,3-dihydroxybiphenyl 1,2-dioxygenase from Dyella ginsengisoli LA-4
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论文类型：期刊论文
发表时间：2009-03-01
发表刊物：FEMS MICROBIOLOGY LETTERS
收录刊物：SCIE、PubMed、Scopus
卷号：292
期号：2
页面范围：231-239
ISSN号：0378-1097
关键字：Dyella ginsengisoli; 2; 3-dihydroxybiphenyl 1; 2-dioxygenase; kinetic parameters; enzyme-substrate complex
摘要：A bphC gene (915 bp) encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC) was amplified by PCR from Dyella ginsengisoli LA-4, which was heterologously expressed in Escherichia coli. The purified His-Tag BphC was able to catalyze the meta-cleavage reaction of the dihydroxylated aromatic rings. According to the specificity constant (K-cat/K-m) of BphC_LA-4, the specificity of BphC_LA-4 was determined in the following order: 2,3-dihydroxybiphenyl > 3-methylcatechol > catechol > 4-chlorocatechol > 4-methylcatechol. The experimental data were consistent with the prediction of enzyme-substrate complexes. The highest specific activity of BphC_LA-4 was 118.3 U mg(-1) for 2,3-dihydroxybiphenyl.