Release Time:2019-03-11  Hits:

Indexed by: Journal Article

Date of Publication: 2012-01-01

Journal: BIOTECHNOLOGY LETTERS

Included Journals: PubMed、EI、SCIE

Volume: 34

Issue: 1

Page Number: 117-123

ISSN: 0141-5492

Key Words: 2,3-Dihydroxybiphenyl-1,2-dioxygenase; Homology modeling; Metagenome; Molecular docking

Abstract: A 2,3-dihydroxybiphenyl-1,2-dioxygenase gene (designated as bphC_meta) was identified in activated sludge metagenome by PCR. This gene shared 99% sequence identity with BphC from Burkholderia xenovorans LB400. The enzyme was purified from recombinant Escherichia coli with a subunit molecular mass of 32 +/- A 1 kDa. It was optimally active at pH 9.0 and 40A degrees C, using 2,3-dihydroxybiphenyl as a substrate. Activity toward substituted catechols was: 2,3-dihydroxybiphenyl > 3-methylcatechol > catechol > 4-chlorocatechol (4-methylcatechol). The prediction made by molecular docking was consistent with the kinetic experimental data, and further explained the substrate preference of BphC_meta. The present study could pave the way for the improved understanding and application of BphCs derived from metagenomes.