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Date of Publication:2008-01-01

Journal:大连海事大学学报

Volume:34

Issue:2

Page Number:37-40

ISSN No.:1006-7736

Abstract:An enterobacter sp.CB-2 isolated from the activated sludge samples was used for microbial degradation experiment on chlorobenzene to research characteristics of microbial degradation and growth in chlorobenzene. Experimental results show that enterobacter sp.CB-2 could degrade chlorobenzene in mineral medium containing 100 mg/L chlorobenzene, with degradation efficiency reaching 81.2% within 24 hours. Enterobacter sp.CB-2 has high resistance to mercuric chloride, which the highest resistance without induction is 20 mg/L of mercuric chloride. Enterobacter sp.CB-2 is well-tolerated in chlorobenzene with 500 mg/L, and it can also be used to degrade some other substrates. Moreover, to confirm the location of degradation gene, plasmid elimination of enterobacter sp.CB-2 was performed with sodium benzoate. Experiment results confirm that the plasmid is related to the characteristic of chlorobenzene degradation, but not to antibiotic resistance.

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