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Indexed by:Journal Papers

Date of Publication:2019-06-01

Journal:MOLECULES

Included Journals:SCIE、PubMed

Volume:24

Issue:11

ISSN No.:1420-3049

Key Words:2-microglobulin; VHH; formylglycine-generating enzyme; hemodialysis

Abstract:Dialysis-related amyloidosis (DRA), which has been widely recognized to be associated with the accumulation of 2-microglobulin (2-m) in blood, is one of the most common complications in patients receiving long-term dialysis treatment. The most significant side-effect of existing hemodialysis sorbents for the removal of 2-m from blood is the loss of vital proteins due to non-specific adsorptions. Although the traditional antibodies have the capability to specifically remove 2-m from blood, high cost limits their applications in clinics. Single domain antibodies derived from the Camelidae species serve as a superior choice in the preparation of immunoadsorbents due to their small size, high stability, amenability, simplicity of expression in microbes, and high affinity to recognize and interact with 2-m. In this study, we modified the anti-2-m VHH by the formylglycine-generating enzyme (FGE), and then directly immobilized the aldehyde-modified VHH to the amino-activated beads. Notably, the fabrication is cost- and time-effective, since all the preparation steps were performed in the crude cell extract without rigorous purification. The accordingly prepared immunoadsorbent with VHHs as ligands exhibited the high capacity of 2-m (0.75 mg/mL). In conclusion, the VHH antibodies were successfully used as affinity ligands in the preparation of novel immunoadsorbents by the site-specific immobilization, and effectively adsorbed 2-m from blood, therefore opening a new avenue for efficient hemodialysis.