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论文类型：期刊论文

发表时间：2017-06-01

发表刊物：ENGINEERING IN LIFE SCIENCES

收录刊物：Scopus、SCIE、EI

卷号：17

期号：6

页面范围：635-644

ISSN号：1618-0240

关键字：1,3-Propanediol; Klebsiella pneumonia; Metabolite profile; Microbial consortium; Substrate stress

摘要：1,3-Propanediol (1,3-PD) is a versatile bulk chemical and widely used as a monomer to synthesis polymers, such as polyesters, polyethers and polyurethanes. 1,3-PD can be produced by microbial fermentation with the advantages of the environmental protection and sustainable development. Low substrate tolerance and wide by-product profile limit microbial production of 1,3-PD by Klebsiella pneumonia on industrial scale. In this study, microbial consortia were investigated to overcome some disadvantages of pure fermentation by single strain. Microbial consortium named DL38 from marine sludge gave the best performance. Its bacterial community composition was analyzed by 16S rRNA gene amplicon high-throughput sequencing and showed that Enterobacteriaceae was the most abundant family. Compared with three K. pneumonia strains isolated from DL38, the microbial consortium could grow well at an initial glycerol concentration of 200 g/L to produce 81.40 g/L of 1,3-PD with a yield of 0.63 mol/mol. This initial glycerol concentration is twice the highest concentration by single isolated strain and more than the critical value (188 g/L) extrapolated from the fermentation kinetics for K. pneumonia. On the other hand, a small amount of by-products were produced in batch fermentation of microbial consortium DL38, especially no 2,3-butanediol detected. The mixed culture of strain W3, Y5 and Y1 improved the tolerance to glycerol and changed the metabolite profile of single strain W3. The batch fermentation with the natural proportion (W3: Y5: Y1 = 208: 82: 17) was superior to that with other proportions and single strain. This study showed that microbial consortium DL38 possessed excellent substrate tolerance, narrow by-product profile and attractive potential for industrial production of 1,3-PD.