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论文类型：期刊论文

发表时间：2019-08-01

发表刊物：PROCESS BIOCHEMISTRY

卷号：83

页面范围：27-34

ISSN号：1359-5113

关键字：Microbial consortium; 1,3-Propandiol; Crude glycerol; Clostridium butyricum DUT1; Klebsiella pneumoniae DUT2; Escherichia coli DUT3

摘要：In this study, a novel consortia DUT08 isolated from wastewater samples could efficiently covert crude glycerol to 1,3-propanediol (1,3-PDO) under anaerobic and microaerobic conditions. The physiological and biochemical characteristics of DUT08 indicated that it consisted of Clostridium (85.25%), Escherichia (12.54%), and Kiebsiella (0.007%) with the neighbor joining method of 16S rRNA gene sequences. In a batch fermentation under microaerobic conditions without nitrogen supply, 43.20 g/L 1,3-PDO with a yield of 0.39 g/g and a productivity of 0.98 g/(L.h) were obtained. In contrast, 61.49 g/L 1,3-PDO (0.55 g/g and 2.46 g/(L.h)) was produced with 0.2 vvm nitrogen supply for 1 h before and 2 h after inoculation, with increasing 1,3-PDO titer by 42.34%, yield by 41.03% and productivity by 151.02%. Three strains, strict anaerobic microbe of Clostridium butyricum DUT1, facultative microbe of Klebsiella pneumoniae DUT2 and Escherichia coli DUT3 were isolated. The function of E. coli DUT3 was found to deplete oxygen and provide anaerobic environment for C. butyricum DUT1. The function of K. pneumoniae DUT2 was found to not only deplete oxygen but also produce 1,3-PDO. Their modes of interaction were commensal and competitive. These results demonstrated the potential advantage of symbiosis and provided a promising strategy for 1,3-propandiol production.