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孙长凯

Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates


Gender:Male
Alma Mater:第四军医大学
Degree:Doctoral Degree
School/Department:人工智能学院
Discipline:Biomedical Engineering
Business Address:大连理工大学创新园大厦B1202
Contact Information:sunck2@dlut.edu.cn
E-Mail:sunck2@dlut.edu.cn
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Current position: Home >> Scientific Research >> Paper Publications

Synthesis of protocatechuic acid grafted chitosan copolymer: Structure characterization and in vitro neuroprotective potential.

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Indexed by:期刊论文

Date of Publication:2018-04-01

Journal:International journal of biological macromolecules

Included Journals:SCIE、PubMed

Volume:109

Page Number:1-11

ISSN No.:1879-0003

Key Words:Antioxidant,Chitosan,Free radical,Oxidative damage,PC12 cells,Protocatechuic acid

Abstract:Excessive free radicals can cause oxidative damage to human tissues, which results in a variety of diseases. Therefore, the development of antioxidant materials is one of the great projects in biomedical field. In this work, antioxidant protocatechuic acid (PCA) monomers were grafted onto chitosan (CS) backbones to develop a PCA grafted chitosan (PCA-g-CS) antioxidant copolymer via the method of free radical-induced grafting reaction. The formation of covalent bonds between PCA and CS were confirmed by FTIR, 1H NMR, XRD and UV-vis. The antioxidant activity of PCA-g-CS was analyzed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging assays. In addition, the cytotoxicity of PCA-g-CS on neuron-like rat phaeochromocytoma (PC12) cells was evaluated by using MTT assay. The neuroprotective effects against hydrogen peroxide (H2O2) and l-glutamic acid (GLU) induced apoptosis in PC12 cells were also investigated. Our results demonstrated that the PCA-g-CS antioxidant copolymer had the ability to scavenge DPPH and hydroxyl radical in vitro. Furthermore, the PCA-g-CS was biocompatible and had neuroprotective effects against free radical-induced apoptosis in PC12 cells. This PCA-g-CS copolymer is firstly synthesized for neuroprotection and the results suggest the PCA-g-CS may be a potential antioxidant material in the treatment of oxidative damage related diseases. Copyright © 2017 Elsevier B.V. All rights reserved.