![]() |
个人信息Personal Information
教授
博士生导师
硕士生导师
性别:男
毕业院校:中国科学院大连化学物理研究所
学位:博士
所在单位:环境学院
学科:环境科学. 环境工程
办公地点:大连理工大学西部校区环境楼B623
联系方式:tanf@dlut.edu.cn
An efficient method for dephosphorylation of phosphopeptides by cerium oxide
点击次数:
论文类型:期刊论文
发表时间:2008-05-01
发表刊物:Journal of Mass Spectrometry
收录刊物:EI
卷号:43
期号:5
页面范围:628-632
ISSN号:10765174
摘要:In this article, an effective method for dephosphorylation of phosphopeptides by cerium oxide is described. The dephosphorylation activity of cerium oxide was evaluated by two standard phosphopeptides and the phosphopeptides in digests of phosphoprotein α-casein and β-casein. Results showed that the dephosphorylation of all the phosphopeptides was completed in 10 min, and temperature had little effect on the dephosphorylation, the dephosphorylation could be carried out at 0°C, room temperature and 37°C. The dephosphorylation mediated by cerium oxide can be attributed to Lewis acid and nucleophile activations. Advantages of using cerium oxide as catalyst for the dephosphorylation include: safe, simple, high catalytic activity, and no precise control of the treatment temperature. The method is valid for the phosphorylation of Ser, Thr and Tyr, and can be used for phosphoprotein analysis. Copyright © 2007 John Wiley & Sons, Ltd.