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论文类型：期刊论文

发表时间：2018-08-08

发表刊物：ACS APPLIED MATERIALS & INTERFACES

收录刊物：PubMed、SCIE

卷号：10

期号：31

页面范围：26653-26661

ISSN号：1944-8244

关键字：hydrogel composite membrane; continuous protein crystallization; morphology selectivity; nucleation and growth control; lysozyme

摘要：The tailored and robust hydrogel composite membranes (HCMs) with diverse ion adsorption and interfacial nucleation property are prepared and successfully used in the continuous lysozyme crystallization. Beyond the heterogeneous supporter, the HCMs functioning as an interface ion concentration controller and nucleation generator are demonstrated. By constructing accurately controlled nucleation and growth circumstances in the HCM-equipped membrane crystallizer, the target desired morphology (hexagon cube) and brand-new morphology (multiple flower shape) that differ from the ones created in the conventional crystallizer are continuously and repetitively generated with ultrahigh morphology selectivity. These tailored robust HCMs show great potential for improving current approaches to continuous protein crystallization with specific crystal targets from laboratorial research to actual engineering applications.