Indexed by:期刊论文
Date of Publication:2013-10-15
Journal:中华临床医师杂志（电子版）
Issue:20
Page Number:9378-9380
ISSN No.:1674-0785
Key Words:巨噬细胞;一氧化氮;BODIPY型一氧化氮探针
Abstract:目的：对BODIPY型一氧化氮探针检测巨噬细胞内一氧化氮的效果进行初步验证。方法采用免疫荧光染色和共聚焦显微镜对小鼠腹腔渗出液中的巨噬细胞的含量进行测定；采用BODIPY型探针对巨噬细胞内一氧化氮进行荧光染色，并激光扫描共聚焦显微镜对细胞内的荧光强度进行测定。结果液状石蜡诱生小鼠腹腔渗出细胞的CD14与CD68阳性细胞率分别为（32.33±7.27）%与（93.83±4.25）%，巨噬细胞为优势细胞；LPS刺激组与对照组细胞的荧光强度与面积比值分别为57.23±18.65和24.57±10.25， LPS刺激巨噬细胞的荧光强度与面积比值明显高于对照组细胞（P＜0.01）。结论 BODIPY型一氧化氮探针可用于细胞一氧化氮荧光成像和巨噬细胞内一氧化氮检测。
Pre One:Liquid Crystalline Perylene Diimide Outperforming Non liquid Crystalline Counterpart: Higher Power Conversion Efficiencies (PCEs) in Bulk Heterojunction (BHJ) Cells and Higher Electron Mobility in Space Charge Limited Current (SCLC) Devices
Next One:Long-Wavelength, Photostable, Two-Photon Excitable BODIPY Fluorophores Readily Modifiable for Molecular Probes
Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
Gender:Male
Alma Mater:Dalian University of Technology
Degree:Doctoral Degree
School/Department:State Key Laboratroy of Fine Chemicals
Discipline:Applied Chemistry. Fine Chemicals. Biochemical Engineering
Business Address:West Campus E-204
Contact Information:xiaoyi@dlut.edu.cn 0411-84986251
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