Indexed by:期刊论文
Date of Publication:2017-09-05
Journal:CHEMBIOCHEM
Included Journals:Scopus、SCIE、PubMed
Volume:18
Issue:17
Page Number:1762-1769
ISSN No.:1439-4227
Key Words:dyes; pigments; fluorescence; proteins; SNAP-tag; two-photon imaging
Abstract:Genetically encoded technologies provide methods for the specific labeling and imaging of proteins, which is essential to understand the subcellular localization of these proteins and their function. Herein, we employed naphthalimide, an efficient two-photon fluorophore, to develop O-6-benzylguanine (BG) derivatives for specific labeling of subcellular proteins and fluorescent imaging through the SNAP-tag. Three naphthalimide-BG derivatives, TNI-BG, QNI-BG, and ONI-BG, were conveniently synthesized through modular click chemistry in high yields. All of them showed high labeling efficiency with SNAP-tag in solution (approximate to 1-2x10(3)s(-1)m(-1)) and in bacteria. Among them, ONI-BG showed high specificity to diffused, histone H2B and mitochondria COX8A targeted SNAP-tag in mammalian cells. The protein-labeled naphthalimides exhibited high two-photon absorption cross-sections, which indicated their potential application in protein-specific two-photon fluorescent imaging, such as two-photon fluorescent lifetime imaging and two-photon multicolor imaging. Therefore, ONI-BG is a versatile tool that can be used to track subcellular proteins through multiple fluorescent techniques.
Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
Gender:Male
Alma Mater:Dalian University of Technology
Degree:Doctoral Degree
School/Department:State Key Laboratroy of Fine Chemicals
Discipline:Applied Chemistry. Fine Chemicals. Biochemical Engineering
Business Address:West Campus E-204
Contact Information:xiaoyi@dlut.edu.cn 0411-84986251
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