Indexed by:期刊论文

Date of Publication:2016-10-01

Journal:DYES AND PIGMENTS

Included Journals:SCIE、EI、Scopus

Volume:133

Page Number:93-99

ISSN No.:0143-7208

Key Words:Fluorescent probe; Rhodamine spirolactam; Hydrogen bond; pH; Fluorescent imaging; Calorimetric

Abstract:A neutral pH fluorescent probe (Rh-Met) based on rhodamine spirolactam is firstly developed according to the modulation strategy of hydrogen bonds on pKa values. The pKa value of Rh-Met was 6.81 (+/- 0.06) and higher than that of other rhodamine spirolactams known. Rh-Met showed a 240-fold enhancement of fluorescence intensity at 585 nm with attenuation of pH values from 9.7 to 3.5. In addition, Rh-Met displayed an excellent selectivity and reversible response to hydrogen ion. And it was successfully employed for imaging endocellular hydrogen ion in mitochondria and lipid droplets. These results suggested that Rh-Met could be a potential tool to assess pH fluctuation in mitochondria and lipid droplets, and would promote many new opportunities for studying the biological effect of pH in living cells. (C) 2016 Elsevier Ltd. All rights reserved.