Indexed by:Journal Papers

Date of Publication:2019-09-18

Journal:JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Included Journals:SCIE、PubMed、EI

Volume:141

Issue:37

Page Number:14491-14495

ISSN No.:0002-7863

Abstract:Insufficient brightness of fluorophores poses a major bottleneck for the advancement of super-resolution microscopes. Despite being widely used, many rhodamine dyes exhibit sub-optimal brightness due to the formation of twisted intramolecular charge transfer (TICT) upon photoexcitation. Herein, we have developed a new class of quaternary piperazine-substituted rhodamines with outstanding quantum yields (Phi = 0.93) and superior brightness (epsilon x Phi = 8.1 X 10(4) L.mol(-)(1).cm(-1)), by utilizing the electronic inductive effect to prevent TICT. We have also successfully deployed these rhodamines in the super-resolution imaging of the microtubules of fixed cells and of the cell membrane and lysosomes of live cells. Finally, we demonstrated that this strategy was generalizable to other families of fluorophores, resulting in substantially increased quantum yields.