Indexed by:Journal Papers

Date of Publication:2015-09-01

Journal:EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Included Journals:SCIE、Scopus

Volume:2015

Issue:25

Page Number:5563-5571

ISSN No.:1434-193X

Key Words:Fluorescent probes; Fluorescence spectroscopy; Click chemistry; 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene; Fluorescent bioimaging

Abstract:Two new two-photon absorbing near-infrared (NIR) emitting 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives, DDC and SDC, were designed for one- and two-photon fluorescence microscopy imaging. Linear and nonlinear photophysical properties, including UV/Vis absorption, fluorescence, excitation anisotropy, photostability, and two-photon absorption cross sections, of the dyes were investigated to assess the potential of using the new probes as bioimaging agents. Cell viability and colocalization studies of DDC with Lysotracker Red, in COS 7 and HCT 116 cells demonstrated not only low toxicity but also selective targeting of lysosomes. SDC was encapsulated in silica-based nanoparticles for in vitro and ex vivo fluorescence bioimaging. The nanoparticles were modified with an RGD peptide to target (V3) integrin. The nanoprobe exhibited good biocompatibility and highly selective uptake in (V3) integrin-overexpressing cancers.