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论文类型： 期刊论文

发表时间： 2017-10-01

发表刊物： CHINESE CHEMICAL LETTERS

收录刊物： SCIE、Scopus

卷号： 28

期号： 10,SI

页面范围： 2019-2022

ISSN号： 1001-8417

关键字： DNA damage; Ratiometric sensor; Fluorescenct imaging; Nucleus labeling; Naphthalimide

摘要： A ratiometric fluorescent sensor (Hoe-NI) was developed by connecting a nucleus targeted Hoechst unit to a naphthalimide dye via "click chemistry". The sensor achieves high specific nucleus labeling with wash-free staining method in various kinds of living cells. The fluorescence ratio of the two emission bands (450 nm for Hoechst and 505 nm for naphthalimide) is changed sensitively to the variation of DNA concentrations, which provides the quantitative information in the processes of DNA damage induced by hydroxyl radicals and antitumor drug. Therefore, Hoe-NI is a recommendable sensor for the monitoring of nuclear DNA damage that reveals the health status of cells. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.