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论文类型： 期刊论文

发表时间： 2016-10-01

发表刊物： DYES AND PIGMENTS

收录刊物： SCIE、EI、Scopus

卷号： 133

页面范围： 93-99

ISSN号： 0143-7208

关键字： Fluorescent probe; Rhodamine spirolactam; Hydrogen bond; pH; Fluorescent imaging; Calorimetric

摘要： A neutral pH fluorescent probe (Rh-Met) based on rhodamine spirolactam is firstly developed according to the modulation strategy of hydrogen bonds on pKa values. The pKa value of Rh-Met was 6.81 (+/- 0.06) and higher than that of other rhodamine spirolactams known. Rh-Met showed a 240-fold enhancement of fluorescence intensity at 585 nm with attenuation of pH values from 9.7 to 3.5. In addition, Rh-Met displayed an excellent selectivity and reversible response to hydrogen ion. And it was successfully employed for imaging endocellular hydrogen ion in mitochondria and lipid droplets. These results suggested that Rh-Met could be a potential tool to assess pH fluctuation in mitochondria and lipid droplets, and would promote many new opportunities for studying the biological effect of pH in living cells. (C) 2016 Elsevier Ltd. All rights reserved.