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发布时间：2019-03-13

论文类型：期刊论文

发表时间：2016-01-01

发表刊物：RSC ADVANCES

收录刊物：EI、SCIE

卷号：6

期号：75

页面范围：71624-71627

ISSN号：2046-2069

摘要：Bright and photostable fluorophores play a key role in the imaging of specific intracellular molecules. Here we report a facile approach to improve the brightness of naphthalimide fluorophores in water. We synthesized a series of nonplanar and rigid compounds (FM1, FM2, and FM3) by fusing a seven-membered heterocycle onto a naphthalimide skeleton. Compared with classic naphthalimide (FM0), these novel fluorophores exhibited much higher quantum yield values (Phi = 0.60-0.66), and higher molar extinction coefficients in aqueous solution, which elicited superior brightness to FM0. They also showed good photostability, pH-independence, and two-photon microscope (TPM) imaging ability. This new strategy of structural nonplanarity and rigidification provides an efficient way to improve the quantum efficiencies of traditional dyes.