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发布时间：2019-11-04

论文类型：期刊论文

发表时间：2019-11-15

发表刊物：ANALYTICA CHIMICA ACTA

收录刊物：PubMed、EI、SCIE

卷号：1082

页面范围：116-125

ISSN号：0003-2670

关键字：Fluorescent probe; Six-membered rhodamine; Hypochlorite detection; Lysosome-targetable; Living cells

摘要：A novel six-membered rhodamine-based fluorescent probe (6G-ClO) was developed from 2-formyl rhodamine (6G-CHO) and used for hypochlorite detection in water and HUVEC cells. Different from planar penta cycle of rhodamine spirolactam, there was a twist six-membered spirocyclic hydrazone in 6G-ClO optimized by Gaussian software at DET/B3LYP/6-31G(d) level. The high selectivity, high sensitivity and fast response of 6G-ClO towards ClO- would be attributed to the twist six-membered spirocycle. Test-strip prepared with 6G-ClO was successfully used to semi-quantitatively indicate the concentration of ClO- in water. 6G-ClO can also quantitatively detect the concentration of ClO- in tap water and swimming pool water. The detection limit of 6G-ClO was as low as 12 nM. The co-localization staining of HUVEC cells further verified that 6G-ClO could specifically accumulate in lysosomes and capture exogenous/endogenous ClO- in living lysosomes. 6G-ClO would be a practical probe for real-time monitoring of ClO- in the biological and real water samples. (C) 2019 Elsevier B.V. All rights reserved.