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论文类型： 期刊论文

发表时间： 2015-09-01

发表刊物： EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

收录刊物： SCIE、Scopus

卷号： 2015

期号： 25

页面范围： 5563-5571

ISSN号： 1434-193X

关键字： Fluorescent probes; Fluorescence spectroscopy; Click chemistry; 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene; Fluorescent bioimaging

摘要： Two new two-photon absorbing near-infrared (NIR) emitting 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives, DDC and SDC, were designed for one- and two-photon fluorescence microscopy imaging. Linear and nonlinear photophysical properties, including UV/Vis absorption, fluorescence, excitation anisotropy, photostability, and two-photon absorption cross sections, of the dyes were investigated to assess the potential of using the new probes as bioimaging agents. Cell viability and colocalization studies of DDC with Lysotracker Red, in COS 7 and HCT 116 cells demonstrated not only low toxicity but also selective targeting of lysosomes. SDC was encapsulated in silica-based nanoparticles for in vitro and ex vivo fluorescence bioimaging. The nanoparticles were modified with an RGD peptide to target (V3) integrin. The nanoprobe exhibited good biocompatibility and highly selective uptake in (V3) integrin-overexpressing cancers.