点击次数：

发布时间：2019-03-09

论文类型：期刊论文

发表时间：2014-12-20

发表刊物：ACS APPLIED MATERIALS & INTERFACES

收录刊物：Scopus、PubMed、EI、SCIE

卷号：6

期号：23

页面范围：21669-21676

ISSN号：1944-8244

关键字：lysosome; probe; near-infrared; fluorescence imaging

摘要：A new borondipyrromethene-based lysosome tracker, Lyso-NIR, is facilely synthesized. Besides the intensive near-infrared (NIR) fluorescence and high photostability, Lyso-NIR shows the capability to stably localize in lysosomes, which is independent of the local pH. Lyso-NIR does not have the problematic alkalization effect suffered by the commonly used lysotrackers; thus, it shows ignorable cytotoxicity and slightly affects normal physiological functions of lysosomes. The above advantages of Lyso-NIR make it feasible to track lysosomes dynamic changes in a relatively long time during the full cellular processes such as apoptosis, heavy metal stimulation, and endocytosis, as is demonstrated in this work. Moreover, Lyso-NIRs narrow NIR emission at 740 nm with a full width at half-maximum smaller than 50 nm makes it easy to avoid the crosstalk with the emissions from other common fluorescent probes, which strengthens Lyso-NIRs competitiveness as a standard lysosome tracker for multicolor bioimaging.