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论文类型： 期刊论文

发表时间： 2013-02-27

发表刊物： JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录刊物： SCIE、EI、PubMed、Scopus

卷号： 135

期号： 8

页面范围： 2903-2906

ISSN号： 0002-7863

摘要： We have developed Lyso-V, the first fluorescent probe of lysosomal viscosity. Because of its lysosome-actived fluorescence characteristics, Lyso-V has proved to be an ideal lysosomal tracer with high spatial and temporal resolution under laser confocal microscopy. More importantly, Lyso-V shows its practical applicability in real-time quantification of lysosomal viscosity changes in live cells through fluorescence lifetime imaging microscopy.