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发表时间：2022-10-09

发表刊物：分析化学

卷号：47

期号：07

页面范围：1021-1028

ISSN号：0253-3820

摘要：The simultaneous and sensitive detection of metabolites of DNA ( uric acid ( UA) , xanthine ( XA) and hypoxanthine ( HX) ) is of great significance for the early diagnosis and prevention of related diseases caused by abnormal metabolism. In this study , hydroxyl functionalized metal-organic framework ( OH-MOFs) and electrochemically reduced graphene oxide ( ERGO ) nano-functional interface was designed and constructed based on the synergic effect of Fe(III) terephthalate OH-MOFs ( OH-MIL-101 ( Fe ) and ERGO for simultaneous detection of UA, XA and HX in blood. Nano-composites were prepared by ultrasonic mixing method , and were characterized by X-ray diffraction ( XRD) , Fourier transform infrared ( FT-IR) and UV-Vis spectroscopic methods. By casting the surface of glassy carbon electrode ( GCE ) with the composite and electrochemical reduction of the modified electrode, OH-MOFs-ERGO/GCE was obtained. Under the optimum conditions , the oxidation peak currents of UA , XA and HX were linearly correlated to their concentrations in the ranges of 0. 20-1150 mu mol/L , 0. 15 -800 mu mol/L , and 0. 40-600 mu mol/L , respectively. The detection limits for UA , XA and HX were 0. 12 , 0. 10 and 0. 20 mu mol/L ( S/N= 3) , respectively. The recoveries of UA, XA and HX in real serum samples were between 99. 5% and 105. 8%. The proposed electrode was expected to provide a simple and easy detection method for the physiological and pathological study of purine metabolism.

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