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个人信息Personal Information
副教授
博士生导师
硕士生导师
性别：男
毕业院校：中国协和医科大学
学位：博士
所在单位：生物工程学院
学科：生物化工. 微生物学. 微生物与生化药学
办公地点：辽宁省大连市高新园区凌工路2号大连理工大学西部校区生物工程学院309室
联系方式：辽宁省大连市高新园区凌工路2号大连理工大学生物工程学院
电子邮箱：yshdong@dlut.edu.cn
Combination of flavonoids from Oroxylum indicum seed extracts and acarbose improves the inhibition of postprandial blood glucose: In vivo and in vitro study
点击次数：
论文类型：期刊论文
发表时间：2017-07-01
发表刊物：BIOMEDICINE & PHARMACOTHERAPY
收录刊物：SCIE、PubMed、Scopus
卷号：91
页面范围：890-898
ISSN号：0753-3322
关键字：Oroxylum indicum; Acarbose; Synergism; Postprandial blood glucose; alpha-Glucosidase; Baicalein
摘要：The combined effect of Oroxylum indicum seed extracts (OISE) or major flavonoids from OISE and acarbose on reducing postprandial blood glucose (PBG) levels was investigated in vitro and in vivo. In vitro, the IC50 values of OISE and baicalein against alpha-glucosidase were 43.4 + 0.731 mu g mL(-1) and 25.9 + 0.412 mu g mL(-1) respectively. A combination of acarbose with OISE or baicalein synergistically inhibited rat intestinal alpha-glucosidase. The combination index (CI) values for acarbose with OISE ranged from 0.33 to 0.75, suggesting a synergistic but not additive effect. OISE was determined to be a non-competitive inhibitor of maltose-hydrolyzing activity. In vivo, OISE were administered to normoglycemic and diabetic mice, either alone or in combination with acarbose. At doses between 50 and 200 mg kg(-1), OISE enhanced the efficacy of acarbose by up to 5-fold. These results demonstrated that OISE enhances the efficacy of acarbose in vivo, and that the combination of OISE and acarbose displayed a synergistic effect in vitro. Therefore, OISE can be used to design dietary supplements to treat diabetes. (C) 2017 Published by Elsevier Masson SAS.